ACID CARDIOLIPIN AND AN IMPROVED METHOD FOR THE PREPARATION OF CARDIOLIPIN FROM BEEF HEART
نویسندگان
چکیده
منابع مشابه
Acid Cardiolipin and an Improved Method for the Preparation of Cardiolipin from Beef Heart
In a previous report (1) the isolation of a new phospholipid from beef heart was described. The name “cardiolipin” was suggested for this substance, which is of particular interest in connection with serodiagnostic tests for syphilis (2). The original method of preparation was tedious and involved considerable losses. An improved and simplified method is here described. The development of the n...
متن کاملNovel preparation of cardiolipin from beef heart.
A new method is described for the isolation of beef heart cardiolipin. A lipid-protein complex, rich in cardiolipin, is obtained by a one-step solvent fractionation of the tissue total lipid extract. Cardiolipin in the complex is largely freed of protein by salt denaturation and is further purified by gel filtration on Sephadex LH-20 followed by column chromatography on bicarbonate-treated sili...
متن کاملActivation of beef-heart cytochrome c oxidase by cardiolipin and analogues of cardiolipin.
Beef-heart cytochrome c oxidase lacking endogenous lipids can be prepared by cholate-mediated exchange with dimyristoylphosphatidylcholine (Powell, G. L., Knowles, P. F. and Marsh, D. (1985) Biochim. Biophys. Acta 816, 191-194). These preparations retained practically no endogenous cardiolipin (less than 0.19 mol cardiolipin per mol of oxidase) but in Tween 80 they retained unaltered electron t...
متن کاملCardiolipin biosynthesis in the isolated heart.
The pathway for the biosynthesis of new cardiolipin was investigated in the isolated perfused intact rat heart. Isolated rat hearts were perfused in the Langendorff mode for up to 60 min with Krebs-Henseleit buffer containing 0.1 microM [U-14C]glycerol. Analysis of radioactivity incorporated into phospholipids in the organic phase revealed an increase in radioactivity incorporated into phosphat...
متن کاملAn investigation into the use of cardiolipin antigens. II. Complement-fixing properties of cardiolipin Wassermann antigen.
Complement-Fixing Properties of Cardiolipin This reagent was examined using two ranges of 0-6 per cent. to 0 0045 per cent. One range was suspended in saline at a dilution of 1 in 50 and the other at 1 in 200. No fixation of complement was obtained when using syphilitic sera. Mixtures of cardiolipin and lecithin were next tested for their complement-fixing ability in the presence of a suitable ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1944
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)71976-5